Rotavirus Specific Neutra™ Antibody Products

Creative Biolabs provides high-quality Rotavirus antibodies designed to meet the demanding needs of your research projects.

• Our antibodies deliver the specificity and sensitivity required for a wide range of applications, ensuring reliable and reproducible results.
• We understand that accurate and consistent data is paramount in scientific research, and our antibodies are developed and validated to meet these critical requirements.
• Whether you are working on developing new diagnostic assays, investigating the pathogenesis of Rotavirus infection, or exploring potential therapeutic interventions, our antibodies provide the precision and performance you need.

This commitment to quality helps to minimize variability and increase confidence in your experimental outcomes, ultimately accelerating your research progress and contributing to more meaningful findings.

Contact our team to get an inquiry now!

Introduction of Rotavirus

Rotavirus, the primary etiological driver of severe pediatric enteric inflammation in children under five years, instigates a clinical triad of projectile emesis, profuse secretory diarrhea, and electrolyte derangement. This fulminant pathophysiological trajectory precipitates hypovolemic shock and small intestinal brush border atrophy, mandating urgent parenteral rehydration—particularly in regions with fragmented healthcare infrastructure where case-fatality rates surge 20-fold absent intervention.

Fig. 1 Rotavirus Reconstruction.Distributed under CC BY 3.0, from Wiki, without modification

Taxonomically nested within Reoviridae, these non-enveloped pathogens (~70 nm diameter) encapsulate an 11-segment dsRNA genome within a triple-layered capsid. The outermost tier, critical for cellular invasion, incorporates two viral glycoproteins: VP4 (protease-cleaved spike) and VP7 (calcium-binding glycoprotein). These surface antigens govern the P/G genotypic classification system, with VP4’s hemagglutinin activity and VP7’s T-cell epitopes jointly directing strain-specific neutralizing antibody responses. Crucially, their antigenic drift facilitates evasion of humoral immunity despite vaccine-induced cross-reactive immunity.

While VP4/VP7-specific antibodies dominate neutralizing activity post-infection or live-attenuated vaccination, nonstructural proteins like NSP4 synergistically modulate enterotoxic effects. The intermediate capsid, composed of VP6 hexamers, exhibits conserved epitopes driving robust IgA/IgG responses—though whether these antibodies sterically hinder viral uncoating or simply mark recent exposure remains contentious. Recent crystallographic analyses reveal VP6’s metastable trimers undergo pH-dependent conformational shifts during endosomal escape, suggesting potential protective roles beyond serological biomarkers.

Antibodies against Rotavirus

The protective response to rotavirus (RVA) infection and illness is now understood to involve both antibody-mediated and cell-mediated mechanisms, both of which are critical for resolving active infection and conferring future protection. The host's initial encounter with RVA primarily elicits a specific, serum-neutralizing antibody response. Subsequent infections, however, trigger a broader, more diverse response. In humans, local gut immunity, especially IgA antibodies, is crucial for protection. Furthermore, safeguarding children from rotavirus-induced diarrhea appears to be serotype-specific and linked to levels of neutralizing antibodies directed at matching viruses, with local IgA antibodies likely having a significant function.

Fig. 2 Characterization of VP6 specific and Wa HRV specific chicken egg yolk IgY Abs.¹

Studies in germ-free pigs infected with HRV have revealed that those recovering from a severe HRV infection completely clear the virus upon re-exposure. The degree of protection in these pigs was positively related to the quantity of IgA antibody-secreting cells and B cell responses in the intestinal lamina propria. Mice serve as another experimental model for investigating RV infection. Murine RV can cause illness in mouse pups, but adult mice are resistant to RV diarrhea, only exhibiting viral shedding. In adult mice, the production of IgG and IgA antibodies, whether systemic or mucosal, is crucial for clearing rotavirus (RV) infections. Studies involving mice with different deficiencies in their immune systems have shown that various antibody-based and innate immune mechanisms work together to provide protection against RV infection. Administering virus-neutralizing antibodies orally presents a promising strategy for providing passive protection against gastrointestinal pathogens in both humans and animals. Investigations using immune bovine colostrum have shown that this preventive passive treatment markedly decreased the occurrence of RVA gastroenteritis outbreaks in children and the duration of RVA-associated diarrhea.

Why Choose Us?

Creative Biolabs is a trusted partner for the provision of high-quality Rotavirus antibodies. We offer several key advantages:

• Unparalleled Specificity and Sensitivity: Our antibodies are generated and rigorously validated to ensure they conform to the highest standards of specificity and sensitivity, thereby minimizing off-target binding and maximizing assay performance.
• Customizable Solutions: We offer a comprehensive range of antibody formats and possess the capability to tailor our services to meet specific project requirements, including custom antibody development, labeling, and conjugation.
• Expertise and Experience: With substantial experience in antibody development and production, our team of scientists possesses the expertise to deliver exceptional results.
• Rigorous Quality Control: We adhere to strict quality control procedures throughout the entire process, from antigen design to final product testing, ensuring the reliability and reproducibility of our antibodies.

For more information about Creative Biolabs' rotavirus antibodies and services, please contact us.

REFERENCE

1. Vega, Celina G., et al. "IgY antibodies protect against human Rotavirus induced diarrhea in the neonatal gnotobiotic piglet disease model." (2012): e42788. Distributed under Open Access license CC BY 4.0, without modification.