pagC Specific Neutra™ Antibody Products

Are you struggling with inconsistent results in targeting virulence factors or delays in antibacterial therapeutic development? Creative Biolabs' pagC specific Neutra™ antibody products accelerate your research with high-affinity, structurally validated antibodies, enabling precise neutralization of pagC-mediated pathogenicity to streamline drug discovery.

Introduction to pagC

Virulence membrane protein pagC is a critical outer membrane protein predominantly expressed in Gram-negative bacterial pathogens, including Salmonella and E. coli. It facilitates host cell invasion by mediating bacterial adherence and immune evasion. pagC is regulated by the PhoP/PhoQ two-component system under low magnesium or acidic conditions, making it essential for intracellular survival and systemic infection.

• Structure

pagC adopts a β-barrel fold with eight transmembrane strands and a surface-exposed loop critical for host-pathogen interactions. Structural studies reveal that its N-terminal domain undergoes pH-dependent conformational changes, enabling reversible binding to host receptors. Dimerization of pagC stabilizes its integration into the bacterial membrane, enhancing resistance to antimicrobial peptides and complement-mediated lysis.

Fig.1 Conformation of the pH response motif of pagC.¹

• Related Signaling Pathways

pagC activates host Toll-like receptor 4 (TLR4) signaling by mimicking endogenous ligands, triggering NF-κB and MAPK pathways. This results in excessive pro-inflammatory cytokine production, contributing to sepsis and tissue damage. Simultaneously, pagC inhibits autophagy by destabilizing the LC3-P62 complex, prolonging bacterial survival in macrophages.

• Pathologies

pagC is directly linked to invasive bacterial infections, including typhoid fever, urinary tract infections, and bloodstream infections. Its role in biofilm formation exacerbates antibiotic resistance, particularly in hospital-acquired infections caused by multidrug-resistant strains.

Applications of Anti-pagC Neutralizing Antibodies

• Therapeutic Development Against Resistant Infections

Anti-pagC antibodies neutralize bacterial adhesion and biofilm formation, offering a synergistic approach with existing antibiotics. In vivo trials show enhanced bacterial clearance in systemic infections, particularly in immunocompromised hosts, offering a critical strategy to combat multidrug-resistant Salmonella and other Gram-negative pathogens.

• Diagnostic Tools for Rapid Pathogen Detection

pagC-specific antibodies are utilized in lateral flow assays and ELISA to detect low-abundance bacterial antigens in clinical samples, enabling early diagnosis and targeted treatment, significantly reducing the time required for pathogen identification compared to traditional culture methods.

• Mechanistic Studies of Bacterial Pathogenesis

Researchers employ these antibodies to map pagC-host receptor interactions via cryo-EM and surface plasmon resonance, guiding the design of small-molecule inhibitors, thereby illuminating the key molecular mechanisms by which the bacteria establish persistent infection.

• Vaccine Development

Anti-pagC antibodies serve as biomarkers to evaluate vaccine efficacy in preclinical models, correlating antibody titers with protective immunity, accelerating the selection of the most promising vaccine candidates for clinical progression.

Our Anti-pagC Antibodies

Neutralizing antibodies against pagC disrupt its functional domains:

- High Specificity: Engineered to bind the surface-exposed loop, blocking receptor interactions and bacterial internalization.

- Cross-Species Efficacy: Validated against pagC homologs in Salmonella enterica and Klebsiella pneumoniae.

- Therapeutic Potential: Preclinical studies demonstrate reduced bacterial load and inflammation in sepsis models following antibody administration.

Creative Biolabs offers pagC-specific antibodies with unmatched specificity and robust validation for infection research and therapeutic development.

Contact our team today to discuss customized solutions for your antibacterial projects.

REFERENCE

1. Dehinwal, Ruchika, et al. "A pH-sensitive motif in an outer membrane protein activates bacterial membrane vesicle production." Nature Communications 15.1 (2024): 6958. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.1038/s41467-024-51364-z