Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize α10β1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Human |
Immunogen | Recombinant alpha10 I-domain |
Isotype | IgG2a kappa |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
Application | ELISA; FC |
Application Notes | The antibody is recommended for detection of α10β1 by ELISA, FC assays. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | α10β1 |
Alternative Name | Integrin α10β1; Integrin alpha10beta1; Alpha chain associated with beta 1 subunit and alpha 10 of integrin; ITGA10; integrin; alpha 10; PRO827; integrin alpha-10; anti-Integrin alpha 10; ITGB1; integrin; beta 1 (fibronectin receptor; beta polypeptide; antigen CD29 includes MDF2; MSK12); CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA; integrin beta-1; glycoprotein IIa; integrin VLA-4 beta subunit; very late activation protein; beta polypeptide |
Research Area | Signal Pathway |