| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Adeno-associated virus Capsid protein VP1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Adeno-associated virus (AAV) |
| Immunogen | AAV Capsid protein VP1 |
| Isotype | IgG3 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | DB; WB; ELISA; FuncS |
| Application Notes | The antibody is recommended for detection of AAV VP1 by WB, ELISA, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-AAV VP1 Monoclonal Antibody (V3S-0522-YC7613) in DB
Dot Blot analysis of V3S-0522-YC7613 was performed by coating with AAV2/2 virus particles (6.78E+12).
V3S-0522-YC7613 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 1 Anti-AAV VP1 Monoclonal Antibody (V3S-0522-YC7613) in DB
Figure 2 Anti-AAV VP1 Monoclonal Antibody (V3S-0522-YC7613) in ELISA
ELISA analysis of V3S-0522-YC7613 was performed by coating with AAV2/2 virus particles (6.78E+12). Then blocked with BSA and incubated with V3S-0522-YC7613. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-AAV VP1 Monoclonal Antibody (V3S-0522-YC7613) in ELISA
Figure 3 Recombinant Mouse Anti-AAV VP1 Antibody (V3S-0522-YC7613) in ELISA
ELISA blot analysis of V3S-0522-YC7613 was performed by coating recombinant AAV2/8 particle. Then blocked with BSA and incubated with Recombinant Mouse Anti-AAV VP1 Antibody (A20) (V3S-0522-YC7613). The Goat Anti-Mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Recombinant Mouse Anti-AAV VP1 Antibody (V3S-0522-YC7613) in ELISA
