Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize ATP Binding Cassette Subfamily G Member 2 (Junior Blood Group). The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Human |
Immunogen | The sequence of ABCG2 transmembrane cell surface antigen polypeptide sequence is designed TGEEYLVKQGIDLSP, which is located on the 618-633 amino acid sequence of the carboxy terminus of ABCG2. |
Epitope | in the aa 618-633. |
Isotype | IgG |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
Application | ELISA; WB |
Application Notes | The antibody is recommended for detection of ABCG2 by ELISA, WB assays. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | ABCG2 |
Alternative Name | ATP Binding Cassette Subfamily G Member 2 (Junior Blood Group); ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (Junior Blood Group); Placenta-Specific ATP-Binding Cassette Transporter; Mitoxantrone Resistance-Associated Protein; Breast Cancer Resistance Protein; Urate Exporter; CDw338; BCRP1; ABCP; BCRP; MXR; Multi Drug Resistance Efflux Transport ATP-Binding Cassette Sub-Family G (WHITE) Member 2; ATP-Binding Cassette, Sub-Family G (WHITE), Member 2; ATP-Binding Cassette Sub-Family G Member 2; |
Gene ID | 9429 |
UniProt | Q9UNQ0 |
Research Area | Signal Pathway |