| Description | The product is a monoclonal antibody that binds specifically to a Staphylococcus spp. autolysin N-acetylmuramoyl-L-alanine amidase catalytic domain. The antibody was raised from the mice immunized with a recombinant form of the entire amidase domain of S. aureus autolysin. The antibody recognizes native Amd and the recombinant form that contains the catalytic region. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Staphylococcus aureus |
| Immunogen | Recombinant form of the entire amidase domain of S. aureus autolysin |
| Epitope | Amidase catalytic domain |
| Affinity | 2.1 nM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA, Inhib |
| Application Notes | The antibody is recommended for detection of AMD by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | AMD |
| Alternative Name | N-acetylmuramoyl-L-alanine amidase; amidase; Amd |