This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Angiopoietin 2. It can be used for Angiopoietin 2 detection in Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry and Neutralization Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality
Monoclonal
Host Species
Human
Target Species
Human
Affinity
The affinity is in picomolar range, about 22 pM, determined by surface plasmon resonance.
Isotype
IgG
Property
Expression Species
HEK293F or CHO Cell line
Conjugation
Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity
>95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin
<1 EU/mg, determined by LAL method
Purification
Purified with Protein A or G affinity chromatography
Sterility
0.2 μM filtered
Formulation
PBS, pH 7.4
Preservation
No preservatives
Stabilizer
No stabilizers
Storage
Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.
