Anti-ASGR1 (ECD domain) Neutralizing Antibody (V3S-0622-YC953) (CAT#: V3S-0622-YC953)

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  • fig1
    Figure 1 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in ELISA
  • fig1
    Figure 2 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in WB
  • fig1
    Figure 3 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in DB

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with ASGR1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen Extracellular domain of human ASGPR HI
Epitope Within the ECD domain.
Isotype IgG1 lambda

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; WB; DB; FuncS
Application Notes The antibody is recommended for detection of ASGR1 by FuncS assay.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target ASGR1
Alternative Name ASGR1; asialoglycoprotein receptor 1; HL-1; ASGPR; ASGPR1; CLEC4H
Research Area Cell Biology

Tested Data

ELISA

Figure 1 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in ELISA

ELISA analysis of V3S-0622-YC953 was performed by coating with human ASGR1 protein (His tag). Then blocked with BSA and incubated with serial dilutions of V3S-0622-YC953. The HRP conjugated anti-human IgG as a secondary antibody diluted at 1/2000.

Figure 1 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in ELISA

WB

Figure 2 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in WB

Western blot analysis of V3S-0622-YC953 was performed by loading human ASGR1 protein, His tag.
The incubation concentration of V3S-0622-YC953 is 2 ng/μL.
The secondary antibody: HRP-Goat anti-human IgG (H+L).

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 2 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in WB

DB

Figure 3 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in DB

Dot Blot analysis of V3S-0622-YC953 was performed by coating with human ASGR1 protein (His tag).
The incubation concentration of V3S-0622-YC953 is 2 μg/mL.
HRP conjugated anti-human IgG as a secondary antibody diluted at 1/2000.

Figure 3 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in DB

For research use only, not directly for clinical use.
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