| Description | This product is a human monoclonal antibody that reacts with ASGR1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Extracellular domain of human ASGPR HI |
| Epitope | Within the ECD domain. |
| Isotype | IgG1 lambda |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FuncS |
| Application Notes | The antibody is recommended for detection of ASGR1 by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ASGR1 |
| Alternative Name | ASGR1; asialoglycoprotein receptor 1; HL-1; ASGPR; ASGPR1; CLEC4H |
| Research Area | Cell Biology |
Figure 1 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in ELISA
ELISA analysis of V3S-0622-YC953 was performed by coating with human ASGR1 protein (His tag). Then blocked with BSA and incubated with serial dilutions of V3S-0622-YC953. The HRP conjugated anti-human IgG as a secondary antibody diluted at 1/2000.
Figure 1 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in ELISA
Figure 2 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in WB
Western blot analysis of V3S-0622-YC953 was performed by loading human ASGR1 protein, His tag.
The incubation concentration of V3S-0622-YC953 is 2 ng/μL.
The secondary antibody: HRP-Goat anti-human IgG (H+L).
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in WB
Figure 3 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in DB
Dot Blot analysis of V3S-0622-YC953 was performed by coating with human ASGR1 protein (His tag).
The incubation concentration of V3S-0622-YC953 is 2 μg/mL.
HRP conjugated anti-human IgG as a secondary antibody diluted at 1/2000.
Figure 3 Human Anti-ASGR Monoclonal Antibody (V3S-0622-YC953) in DB
