Anti-BTN3A1 Neutralizing Antibody (V3S-0522-YC7633) (CAT#: V3S-0522-YC7633)

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  • fig1
    Figure 1 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in WB
  • fig1
    Figure 2 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in ELISA

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Butyrophilin, subfamily 3, member A1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Human butyrophilin, subfamily 3, member A1
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; WB; FC; Block
Application Notes The antibody is recommended for detection of BTN3A1 by FC, Block assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target BTN3A1
Alternative Name BTN3A1; butyrophilin, subfamily 3, member A1; BTF5; BT3.1; CD277; BTN3.1; butyrophilin subfamily 3 member A1; dJ45P21.3 (butyrophilin, subfamily 3, member A1)
Gene ID 11119
UniProt O00481
Research Area Cancer Research

Tested Data

WB

Figure 1 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in WB

Western blot analysis of V3S-0522-YC7633 was performed by loading Recombinant Human BTN3A1 Protein (Lane 1, 0.1 μg; Lane 2, 0.3 μg; Lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC7633 and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.

Figure 1 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in WB

ELISA

Figure 2 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in ELISA

ELISA analysis of V3S-0522-YC7633 was performed by coating with Recombinant Human BTN3A1 Protein. Then blocked with BSA, and incubated with Anti-Human BTN3A1 Antibody (V3S-0522-YC7633). The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in ELISA

For research use only, not directly for clinical use.
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