| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Butyrophilin, subfamily 3, member A1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Human butyrophilin, subfamily 3, member A1 |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; FC; Block |
| Application Notes | The antibody is recommended for detection of BTN3A1 by FC, Block assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in WB
Western blot analysis of V3S-0522-YC7633 was performed by loading Recombinant Human BTN3A1 Protein (Lane 1, 0.1 μg; Lane 2, 0.3 μg; Lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC7633 and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 1 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in WB
Figure 2 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in ELISA
ELISA analysis of V3S-0522-YC7633 was performed by coating with Recombinant Human BTN3A1 Protein. Then blocked with BSA, and incubated with Anti-Human BTN3A1 Antibody (V3S-0522-YC7633). The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-Recombinant BTN3A1 Monoclonal Antibody (V3S-0522-YC7633) in ELISA
