Recombinant Anti-C. botulinum botA (aa 918-1066) Antibody (V3S-0522-YC4255) (CAT#: V3S-0522-YC4255)

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Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Clostridium botulinum Botulinum neurotoxin type A precursor. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Clostridium botulinum
Epitope The epitope contains residues N918, L919, E920, F953, R1061, D1062, T1063, H1064, Y1066.
Isotype IgG


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application FC
Application Notes The antibody is recommended for detection of C. botulinum botA by FC assay.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target C. botulinum botA
Alternative Name C. botulinum botA; Clostridium botulinum Botulinum neurotoxin type A precursor; C. botulinum; Clostridium botulinum; Botulinum neurotoxin type A precursor; botA
UniProt P10845
Research Area Microbiology
Related Disease Flaccid paralytic
For research use only, not directly for clinical use.
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