| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Carbonic anhydrase 9. It can be used for Carbonic anhydrase 9 detection in Western Blot, Enzyme-Linked Immunosorbent Assay and Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Cynomolgus |
| Epitope | The epitope is in the amino acid residues EDLPGEED, corresponding to AA 81-88 and AA 87-94 of CA-IX. |
| Affinity | The affinity is in nanomolar range, about 0.21 nM, determined by surface plasmon resonance. |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | DB; WB; ELISA; FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CA9 |
| Alternative Name | Carbonic Anhydrase IX; Renal Cell Carcinoma-Associated Antigen G250; RCC-Associated Protein G250; RCC-Associated Antigen G250; Carbonate Dehydratase IX; Carbonic Dehydratase; Membrane Antigen MN; P54/58N; CA-IX; CAIX; PMW1; MN; EC 4.2.1.1; G250 |
| Gene ID | 768 |
| UniProt | Q16790 |
| Research Area | Cardiovascular; Signal Pathway |
| Related Disease | Cancer |
Figure 1 Anti-CA9 Monoclonal Antibody (V3S-1022-YC3036) in WB
Western blot analysis of V3S-1022-YC3036 was performed by loading Human CA9 Protein (His Tag) in reduced condition (Lane 1) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC3036 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 1 Anti-CA9 Monoclonal Antibody (V3S-1022-YC3036) in WB
Figure 2 Anti-CA9 Monoclonal Antibody (V3S-1022-YC3036) in DB
Dot Blot analysis of V3S-1022-YC3036 was performed by coating with Human CA9 Protein (His Tag).
V3S-1022-YC3036 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
Figure 2 Anti-CA9 Monoclonal Antibody (V3S-1022-YC3036) in DB
Figure 3 Anti-CA9 Monoclonal Antibody (V3S-1022-YC3036) in ELISA
ELISA analysis of V3S-1022-YC3036 was performed by coating with Human CA9 Protein (His Tag). Then blocked with BSA and incubated with V3S-1022-YC3036. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-CA9 Monoclonal Antibody (V3S-1022-YC3036) in ELISA
