| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize CCR4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant CCR4 protein |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | DB; ELISA; FC; WB |
| Application Notes | The antibody is recommended for detection of CCR4 by ELISA, FC, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Mouse Anti-CCR4 Monoclonal Antibody (V3S-0622-YC2954) in DB
Dot Blot analysis of V3S-0622-YC2954 was performed by coating with human CCR4 protein (His tag).
V3S-0622-YC2954 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 1 Mouse Anti-CCR4 Monoclonal Antibody (V3S-0622-YC2954) in DB
Figure 2 Mouse Anti-CCR4 Monoclonal Antibody (V3S-0622-YC2954) in ELISA
ELISA analysis of V3S-0622-YC2954 was performed by coating with human CCR4 protein (His tag). Then blocked with BSA and incubated with V3S-0622-YC2954. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Mouse Anti-CCR4 Monoclonal Antibody (V3S-0622-YC2954) in ELISA
