| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize CD200 receptor 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human CD200 receptor 1 |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; FuncS |
| Application Notes | The antibody is recommended for detection of CD200R1 by WB, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD200R1 |
| Alternative Name | CD200R1; CD200 receptor 1; OX2R; MOX2R; CD200R; HCRTR2; cell surface glycoprotein CD200 receptor 1; MOX2 receptor; CD200 cell surface glycoprotein receptor; cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200 |
| Gene ID | 131450 |
| UniProt | Q8TD46 |
| Research Area | Immunology; Microbiology |
Figure 1 Anti-CD200R1 Monoclonal Antibody (V3S-0522-YC7079) in SEC-HPLC
The purity of V3S-0522-YC7079 was greater than 94% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 25 µl
Figure 1 Anti-CD200R1 Monoclonal Antibody (V3S-0522-YC7079) in SEC-HPLC
Figure 2 Anti-CD200R1 Monoclonal Antibody (V3S-0522-YC7079) in ELISA
ELISA analysis of V3S-0522-YC7079 was performed by coating with Recombinant Human CD200R1 Protein. Then blocked with BSA, and incubated with Anti-Human CD200R Antibody (V3S-0522-YC7079). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-CD200R1 Monoclonal Antibody (V3S-0522-YC7079) in ELISA
