| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize CD24 molecule. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Isotype | IgG2a |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; FC |
| Application Notes | The antibody is recommended for detection of CD24 by FC assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC5143 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates about 180 kDa.
Figure 1 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in SDS-PAGE
Figure 2 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in WB
Western blot analysis of V3S-0522-YC5143 was performed by loading 2 µg onto a 12% Tris-HCl polyacrylamide gel in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with Goat Anti-Mouse IgG HRP secondary antibody at a dilution of 1:8,000 for 75 minutes. Chemiluminescent detection was performed.
Figure 2 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in WB
Figure 3 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in SEC-HPLC
The purity of V3S-0522-YC5143 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 3 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in SEC-HPLC
Figure 4 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in WB
Western blot analysis of V3S-0522-YC5143 was performed by loading Recombinant Human CD24, His tag in non-reduced (lane 1, 0.5 μg) and reduced condition (lane 2, 0.5 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC5143 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 4 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in WB
Figure 5 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in ELISA
ELISA analysis of V3S-0522-YC5143 was performed by coating with Recombinant Human CD24, His tag. Then blocked with BSA, and incubated with Anti-Human TIM3 Antibody (V3S-0522-YC5143). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 5 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in ELISA
