Recombinant Anti-CD24 Antibody (V3S-0522-YC5143) (CAT#: V3S-0522-YC5143)

Figure 1 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC5143 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates about 180 kDa.

Figure 2 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in WB

Western blot analysis of V3S-0522-YC5143 was performed by loading 2 µg onto a 12% Tris-HCl polyacrylamide gel in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with Goat Anti-Mouse IgG HRP secondary antibody at a dilution of 1:8,000 for 75 minutes. Chemiluminescent detection was performed.

Figure 3 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in SEC-HPLC

The purity of V3S-0522-YC5143 was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 4 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in WB

Western blot analysis of V3S-0522-YC5143 was performed by loading Recombinant Human CD24, His tag in non-reduced (lane 1, 0.5 μg) and reduced condition (lane 2, 0.5 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC5143 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.

Figure 5 Anti-Human CD24 Monoclonal Antibody (V3S-0522-YC5143) in ELISA

ELISA analysis of V3S-0522-YC5143 was performed by coating with Recombinant Human CD24, His tag. Then blocked with BSA, and incubated with Anti-Human TIM3 Antibody (V3S-0522-YC5143). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.