Anti-CD24 Neutralizing Antibody (V3S-0622-YC1243) (CAT#: V3S-0622-YC1243)

Figure 1 Anti-CD24 Monoclonal Antibody (V3S-0622-YC1243) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC1243 in non-reduced (lane 2, 2 μg) and reduced (lane 1, 3.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Anti-CD24 Monoclonal Antibody (V3S-0622-YC1243) in SEC-HPLC

The purity of V3S-0622-YC1243 was greater than 98% as determined by HPLC.

Figure 3 Anti-CD24 Monoclonal Antibody (V3S-0622-YC1243) in WB

Western blot analysis was performed by loading recombinant human CD24 (RPB143Hu01) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human CD24 Antibody (V3S-0622-YC1243) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Non-Reducing Antigen (0.05 μg)
Lane 2: Reducing Antigen (0.05 μg)

Figure 4 Anti-CD24 Monoclonal Antibody (V3S-0622-YC1243) in ELISA

ELISA analysis of V3S-0622-YC1243 was performed by coating with recombinant human CD24 (RPB143Hu01). Then blocked with BSA and incubated with Anti-Human CD24 Antibody (V3S-0622-YC1243) at a starting concentration of 1 ng/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.