| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for CD27 molecule. It can be used for CD27 molecule detection in Enzyme-Linked Immunosorbent Assay, Flow Cytometry and Blocking. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Rhesus |
| Immunogen | A soluble fusion protein comprising a CD27 extracellular domain fused with an antibody Fc domain (recombinant human CD27-Fc chimeric protein) |
| Epitope | Within the ECD domain. |
| Affinity | The affinity is in nanomolar range, about 0.39 nM, determined by surface plasmon resonance. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB; DB; ELISA; FC; Block |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD27 |
| Alternative Name | CD27 Molecule; Tumor Necrosis Factor Receptor Superfamily, Member 7; T-Cell Activation Antigen CD27; CD27L Receptor; TNFRSF7; T14; Tumor Necrosis Factor Receptor Superfamily Member 7; T Cell Activation Antigen S152; CD27 Antigen; S152. LPFS2; S152; Tp55 |
| Gene ID | 939 |
| UniProt | P26842 |
| Research Area | Immunology |
| Related Disease | Cancer |
Figure 1 Anti-CD27 Monoclonal Antibody (V3S-1022-YC2572) in ELISA
ELISA analysis of V3S-1022-YC2572 was performed by coating with Human PCNA Protein (His Tag). Then blocked with BSA and incubated with V3S-1022-YC2572. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-CD27 Monoclonal Antibody (V3S-1022-YC2572) in ELISA
Figure 2 Anti-CD27 Monoclonal Antibody (V3S-1022-YC2572) in WB
Western blot analysis of V3S-1022-YC2572 was performed by loading Human CD27 Protein (His Tag) in reduced condition Lane 2 and Lane 3 (0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC2572 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 2 Anti-CD27 Monoclonal Antibody (V3S-1022-YC2572) in WB
Figure 3 Anti-CD27 Monoclonal Antibody (V3S-1022-YC2572) in DB
Dot Blot analysis of V3S-1022-YC2572 was performed by coating with Human CD27 Protein (His Tag).
V3S-1022-YC2572 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control, PC: Positive control
Figure 3 Anti-CD27 Monoclonal Antibody (V3S-1022-YC2572) in DB
