| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize CD274 molecule. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Epitope | The antibody was found to bind an epitope within the sequence LKVQHSSYRQREGYPKAEVIWTSSDHQ, which are amino acids 74 to 84 and 158 to 173, respectively, and contacts residues S80, S81, K162 and S169 within residues 74 to 173. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; Block; IHC; WB |
| Application Notes | The antibody is recommended for detection of CD274 by FC, Block, IHC, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-CD274 Monoclonal Antibody (V3S-0522-YC3137) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC3137 in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of proteins (heavy chain and light chain) in different condition migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-CD274 Monoclonal Antibody (V3S-0522-YC3137) in SDS-PAGE
Figure 2 Anti-CD274 Monoclonal Antibody (V3S-0522-YC3137) in WB
Western blot analysis of V3S-0522-YC3137 was performed by loading Recombinant human PD-L1 protein in non-reduced (Lane 1, 0.2 μg) and reduced (Lane 2, 0.2 μg) condition. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC3137 (1 μg/ml) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:5000). Chemiluminescent detection was performed.
Figure 2 Anti-CD274 Monoclonal Antibody (V3S-0522-YC3137) in WB
Figure 3 Anti-CD274 Monoclonal Antibody (V3S-0522-YC3137) in ELISA
ELISA analysis of V3S-0522-YC3137 was performed by coating with Recombinant human PD-L1 protein. Then blocked with BSA, and incubated with V3S-0522-YC3137. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-CD274 Monoclonal Antibody (V3S-0522-YC3137) in ELISA
