Recombinant Anti-CD274 Antibody (V3S-0522-YC3138) (CAT#: V3S-0522-YC3138)

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Datasheet

MSDS

COA

Sub Cat Immunogen Isotype Application Affinity  
V3S-1124-YC141 Recombinant PDL1 IgG ELISA; FC; IHC Inquiry
V3S-0622-YC1259 Recombinant human PD-L1-His tag protein IgG ELISA; FC Inquiry
V3S-0622-YC1275 Purified human B7-H1Ig IgG FC Inquiry
V3S-0622-YC1276 IgG ELISA Inquiry
V3S-0622-YC74 IgG1 IHC Inquiry
V3S-1222-YC421 IgG2a FC; ELISA Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize CD274 molecule. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Recombinant PDL1
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; FC; IHC
Application Notes The antibody is recommended for detection of CD274 by ELISA, FC, IHC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CD274
Alternative Name CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1
Gene ID 29126
UniProt Q9NZQ7
Research Area Immunology
Related Disease Tumor, Immunological Disease, Chronic Infectious Disease
For research use only, not directly for clinical use.
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