| Description | This product is a recombinant Mouse antibody provided by Creative Biolabs. The antibody is specific for CD38. It can be used for CD38 detection in Enzyme-Linked Immunosorbent Assay and Blocking. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Block |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD38 |
| Alternative Name | CD38 Molecule; ADP-Ribosyl Cyclase 1; 2-Phospho-Cyclic-ADP-Ribose Transferase; Cyclic ADP-Ribose Hydrolase 1; 2-Phospho-ADP-Ribosyl Cyclase; NAD(+) Nucleosidase; CD38 Antigen (P45); ADPRC 1; 2-Phospho-ADP-Ribosyl Cyclase/2-Phospho-Cyclic-ADP-Ribose Transferase; Ecto-Nicotinamide Adenine Dinucleotide Glycohydrolase; |
| Gene ID | 952 |
| UniProt | P28907 |
Figure 1 Mouse Anti-CD38 Antibody (V3S-1222-YC376) in ELISA.
ELISA analysis of V3S-1222-YC376 was performed by coating with recombinant human CD38 protein (His Tag). Then blocked with BSA and incubated with mouse anti-CD38 Antibody (V3S-1222-YC376). The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Mouse Anti-CD38 Antibody (V3S-1222-YC376) in ELISA.
Figure 2 Mouse Anti-CD38 Antibody (V3S-1222-YC376) in Western Blot.
Western blot analysis of V3S-1222-YC376 was performed by loading recombinant human CD38 protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1222-YC376 and HRP goat anti-mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Non-Reduced antigen (0.1 ug)
Lane 2: Reduced antigen (0.1 ug)
Figure 2 Mouse Anti-CD38 Antibody (V3S-1222-YC376) in Western Blot.
