Anti-CD40LG Neutralizing Antibody (V3S-0622-YC1332) (CAT#: V3S-0622-YC1332)

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  • fig1
    Figure 1 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in ELISA
  • fig1
    Figure 2 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in WB
  • fig1
    Figure 3 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in DB

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with CD40LG. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen D1.1 cells
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; DB; IF; IP; Neut; FuncS; ELISA; FC; ICC
Application Notes The antibody is recommended for detection of CD40LG by IF, IP, Neut, FuncS, ELISA, FC, ICC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CD40LG
Alternative Name CD40LG; CD40 ligand; HIGM1, IMD3, TNFSF5, tumor necrosis factor (ligand) superfamily, member 5 (hyper IgM syndrome); CD40 antigen ligand; CD40L; CD154; gp39; hCD40L; hyper IgM syndrome; T B cell activati
Gene ID 959
UniProt P29965
Research Area Immunology

Tested Data

ELISA

Figure 1 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in ELISA

ELISA analysis of V3S-0622-YC1332 was performed by coating with Recombinant Human CD40L.
The secondary antibody: HRP-anti-human IgG (H+L)

Figure 1 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in ELISA

WB

Figure 2 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in WB

Western blot analysis of V3S-0622-YC1332 was performed by loading Recombinant Human CD40L (His Tag).
The incubation concentration of V3S-0622-YC1332 is 2 ng/μL.
The secondary antibody: HRP-anti-human IgG (H+L)

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 2 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in WB

DB

Figure 3 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in DB

Dot blot analysis of V3S-0622-YC1332 was performed by loading Recombinant Human CD40L (His Tag).
The incubation concentration of V3S-0622-YC1332 is 2 μg/mL.
The secondary antibody: HRP-anti-human IgG (H+L)

Figure 3 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in DB

For research use only, not directly for clinical use.
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