| Description | This product is a human monoclonal antibody that reacts with CD40LG. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | D1.1 cells |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; DB; IF; IP; Neut; FuncS; ELISA; FC; ICC |
| Application Notes | The antibody is recommended for detection of CD40LG by IF, IP, Neut, FuncS, ELISA, FC, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in ELISA
ELISA analysis of V3S-0622-YC1332 was performed by coating with Recombinant Human CD40L.
The secondary antibody: HRP-anti-human IgG (H+L)
Figure 1 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in ELISA
Figure 2 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in WB
Western blot analysis of V3S-0622-YC1332 was performed by loading Recombinant Human CD40L (His Tag).
The incubation concentration of V3S-0622-YC1332 is 2 ng/μL.
The secondary antibody: HRP-anti-human IgG (H+L)
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in WB
Figure 3 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in DB
Dot blot analysis of V3S-0622-YC1332 was performed by loading Recombinant Human CD40L (His Tag).
The incubation concentration of V3S-0622-YC1332 is 2 μg/mL.
The secondary antibody: HRP-anti-human IgG (H+L)
Figure 3 Anti-Human CD40L Monoclonal Antibody (V3S-0622-YC1332) in DB
