| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize CD8a molecule. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Isotype | IgG2a |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | FC |
| Application Notes | The antibody is recommended for detection of CD8A by FC assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Mouse CD8A Recombinant Antibody (V3S-0522-YC5426) in Western Blot.
Western blot analysis of V3S-0522-YC5426 was performed by loading recombinant mouse CD8A protein onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC5426 and goat anti-mouse IgG-HRP as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 1 Anti-Mouse CD8A Recombinant Antibody (V3S-0522-YC5426) in Western Blot.
Figure 2 Anti-Mouse CD8A Recombinant Antibody (V3S-0522-YC5426) in ELISA.
ELISA analysis of V3S-0522-YC5426 was performed by coating with recombinant mouse CD8A protein. Then blocked with BSA, and incubated with anti-mouse CD8A Antibody (V3S-0522-YC5426). The goat anti-mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-Mouse CD8A Recombinant Antibody (V3S-0522-YC5426) in ELISA.
Figure 3 Anti-Mouse CD8A Recombinant Antibody (V3S-0522-YC5426) in Dot Blot.
Dot blot analysis of V3S-0522-YC5426 was performed by coating with recombinant mouse CD8A protein.
V3S-0522-YC5426 incubation concentration: 1.25 ng/μL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 3 Anti-Mouse CD8A Recombinant Antibody (V3S-0522-YC5426) in Dot Blot.
