| Description | This product is a rat monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing CD8 antigen, alpha chain. It can be used for Cd8a detection in Depletion Assay (Dep), Western Blot (WB). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Rat |
| Target Species | Mouse |
| Immunogen | Mouse CTL clone L3 |
| Isotype | IgG2b kappa |
| Purity | >95%, determined by SDS-PAGE |
| Purification | Protein G purified |
| Formulation | PBS, pH 7.0 |
| Preservation | No preservatives |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | Dep; WB |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Cd8a |
| Alternative Name | BB154331; Ly-2; Ly-35; Ly-B; Lyt-2 |
| Gene ID | 12525 |
| Research Area | Immunology |
Figure 1 Anti-Mouse CD8A Recombinant Antibody (V3S-0822-YC2705) in Western Blot.
Western blot analysis of V3S-0822-YC2705 was performed by loading recombinant mouse CD8A protein onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0822-YC2705 and goat anti-mouse IgG-HRP as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 1 Anti-Mouse CD8A Recombinant Antibody (V3S-0822-YC2705) in Western Blot.
Figure 2 Anti-Mouse CD8A Recombinant Antibody (V3S-0822-YC2705) in ELISA.
ELISA analysis of V3S-0822-YC2705 was performed by coating with recombinant mouse CD8A protein. Then blocked with BSA, and incubated with anti-mouse CD8A Antibody (V3S-0822-YC2705). The goat anti-mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-Mouse CD8A Recombinant Antibody (V3S-0822-YC2705) in ELISA.
Figure 3 Anti-Mouse CD8A Recombinant Antibody (V3S-0822-YC2705) in Dot Blot.
Dot blot analysis of V3S-0822-YC2705 was performed by coating with recombinant mouse CD8A protein.
V3S-0822-YC2705 incubation concentration: 1.25 ng/μL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 3 Anti-Mouse CD8A Recombinant Antibody (V3S-0822-YC2705) in Dot Blot.
