| Description | The antibody specifically recognizes CEACAM5 and CEACAM6 proteins, not CEACAM 1 or CEACAM8. The antibody specifically binds to residues 34-319 of CEACAM5, which shares high sequence identity to CEACAM6. It binds to tumor cells and initiates antibody-dependent cell-mediated cytotoxicity (ADCC) and inhibits cell proliferation. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Epitope | The epitope is not a linear polypeptide, but rather is comprised of at least two polypeptide regions, containing GPDGPTI (amino acids 236-242) and GSYMCQAHNSATGLNRTTVTMITVS (amino acids 295-319), that are separated by 60 amino acids. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | WB; ELISA; FC; IHC; IF; FuncS |
| Application Notes | The antibody is recommended for detection of CEACAM5/CEACAM6 by WB (1/1000-1/5000), ELISA, FC, IHC (1/20-1/200), IF (1/100-1/500), FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CEACAM5/CEACAM6 |
| Alternative Name | Carcinoembryonic antigen related cell adhesion molecule 5/Carcinoembryonic antigen related cell adhesion molecule 6; CEA; NCA; CEAL; CD66c; carcinoembryonic antigen-related cell adhesion molecule 6; Cluster of Differentiation 66c; carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen); normal cross-reacting antigen; CD66e; carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100 |
| Research Area | Cancer Research |
