| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Carcinoembryonic antigen-related cell adhesion molecule 5. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | FuncS |
| Application Notes | The antibody is recommended for detection of CEACAM5 by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CEACAM5 |
| Alternative Name | CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100; |
| Gene ID | 1048 |
| UniProt | A0A024R0K5 |
| Research Area | Cancer Research; Immunology |
| Related Disease | Cancer |
Figure 1 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SDS-PAGE.
SDS-PAGE analysis of V3S-0522-YC5482 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.
Figure 1 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SDS-PAGE.
Figure 2 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SEC-HPLC.
The purity of V3S-0522-YC5482 was greater than 96% as determined by SEC-HPLC.
Figure 2 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SEC-HPLC.
Figure 3 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in Western Blot.
Western blot analysis of V3S-0522-YC5482 was performed by loading human CEACAM5 protein, His tag.
Figure 3 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in Western Blot.
Figure 4 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in ELISA.
ELISA analysis of V3S-0522-YC5482 was performed by coating with human CEACAM5 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC5482. The HRP conjugated anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in ELISA.
