Recombinant Anti-CEACAM5 Antibody (V3S-0522-YC5482) (CAT#: V3S-0522-YC5482)

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  • fig1
    Figure 1 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SDS-PAGE.
  • fig1
    Figure 2 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SEC-HPLC.
  • fig1
    Figure 3 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in Western Blot.
  • fig1
    Figure 4 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in ELISA.

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Carcinoembryonic antigen-related cell adhesion molecule 5. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application FuncS
Application Notes The antibody is recommended for detection of CEACAM5 by FuncS assay.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CEACAM5
Alternative Name CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100;
Gene ID 1048
UniProt A0A024R0K5
Research Area Cancer Research; Immunology
Related Disease Cancer

Tested Data

SDS-PAGE

Figure 1 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SDS-PAGE.

SDS-PAGE analysis of V3S-0522-YC5482 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.

Figure 1 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SDS-PAGE.

SEC-HPLC

Figure 2 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SEC-HPLC.

The purity of V3S-0522-YC5482 was greater than 96% as determined by SEC-HPLC.

Figure 2 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in SEC-HPLC.

WB

Figure 3 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in Western Blot.

Western blot analysis of V3S-0522-YC5482 was performed by loading human CEACAM5 protein, His tag.

Figure 3 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in Western Blot.

ELISA

Figure 4 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in ELISA.

ELISA analysis of V3S-0522-YC5482 was performed by coating with human CEACAM5 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC5482. The HRP conjugated anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Anti-Human CEACAM5 Recombinant Antibody (V3S-0522-YC5482) in ELISA.

For research use only, not directly for clinical use.
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