| Description | This product is a monoclonal antibody which can specifically recognizes CLDN18.2. The epitope of the antibody located within the C-terminal portion of CLDN18.2. The mAb can be used for determining whether cancer cells express CLDN18.2 and diagnosing CLDN18.2 related cancer. The CLDN18 isoform (e.g. CLDN18.1) expressed in these respiratory tissues is not recognized by the antibody 43-14A. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | HIS-tagged CLDN18 C-termal recombinant protein |
| Epitope | The epitope is located in the C-terminal, EVQSYPSKHDYV. |
| Isotype | IgG kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | WB; ELISA; IHC-P |
| Application Notes | /b>Immunohistochemistry assay Immunohistochemistry was performed on slides of 4% buffered formalin fixed paraffin embedded samples. After deparaffinization, all slides were subjected to antigen retrieval by boiling in 10 mM citric acid supplemented with 0.05% Tween-20 (pH 6.0) at 120°C for 10 min, subsequently quenched, blocked and incubated overnight at 4°C with 0.2 to 0.5 μg/mL diagnostic monoclonal mouse anti-CLDN18.2 antibody clone 43-14A. Antibody binding was visualized with horseradish-peroxidase-labeled secondary antibodies and a substrate-chromogen solution. Sections were subsequently counter- stained with Mayer's haematoxylin and subjected to evaluation by the raters. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
