| Description | The antibodies described herein is a recombinant antibody against CLDN18.2 protein. The antibody is capable of detecting CLDN18.2 expressing tumor cells in stomach CA, esophagus CA, pancreas CA and lung CA FFPE tissues. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | HIS-tagged CLDN18 C-termal recombinant protein |
| Epitope | The epitope is located in the C-terminal, EVQSYPSKHDYV. |
| Isotype | IgG kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | WB; ELISA; IHC-P |
| Application Notes | /b>Immunohistochemistry assay Immunohistochemistry was performed on slides of 4% buffered formalin fixed paraffin embedded samples. After deparaffinization, all slides were subjected to antigen retrieval by boiling in 10 mM citric acid supplemented with 0.05% Tween-20 (pH 6.0) at 120°C for 10 min, subsequently quenched, blocked and incubated overnight at 4°C with 0.2 to 0.5 μg/mL diagnostic monoclonal mouse anti-CLDN18.2 antibody clone 35-22A. Antibody binding was visualized with horseradish-peroxidase-labeled secondary antibodies and a substrate-chromogen solution. Sections were subsequently counter- stained with Mayer's haematoxylin and subjected to evaluation by the raters. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
