| Description | This mouse neutralizing antibody is specific against CVB1 capsid protein, termed as 9A3. It binds mainly to the viral VP2 domain of CVB1 capsid protein. 9A3 exhibits potent neutralizing activity against CVB1 and it inhibits CAR binding to the virus in a dose-dependent manner. This product has been determined by Enzyme-linked immunosorbent assay, Immunofluorescence, Bio-layer interferometry and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Coxsackievirus B1 (CVB1) |
| Epitope | 9A3 binds mainly to the viral VP2 domain. |
| Neutralization Mechanism | 9A3 binding footprints closely encircle the canyon region and overlap with the CAR-binding site. |
| Neutralization Breath | The middle dosage (0.1 mg/kg) of 9A3 offered 75% protection against lethal challenge |
| IC50 | 0.376 μg/mL |
| Affinity | 240.5 ± 236.6 nM |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; SPR; IF; Neut |
| Application Notes | This antibody was tested by ELISA, Surface plasmon resonance (SPR), Immunofluorescence (IF) and Neutralization assay. ELISA: mAbs at various concentrations were first incubated at 37°C for 30 min, then combined with the soluble CAR (2 mg/mL) and further incubated at 37°C for 30 min. Surface plasmon resonance (SPR): MAbs were directly immobilized onto a Protein G biosensor chip (Cytiva) using a PBS running buffer. Immunofluorescence (IF): After blocking with 2% bovine serum albumin (BSA) diluted in PBS, cells were incubated with mAbs (5 μg/mL) at 37°C for 1 h. Neutralization assay (Neut): MAbs was 2-fold serially diluted and incubated with an equal volume of CVB1 at 37°C for 1 h. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CVB1 VP2 |
| Alternative Name | Coxsackievirus B1 capsid protein |
| Research Area | Infectious Disease |
| Related Disease | Aseptic meningitis; myocarditis; pancreatitis |
