| Description | This product is a human monoclonal antibody that reacts with DDR1. The antibody exhibites high affinity for DDR1 protein and antagonizes DDR1 signaling. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib |
| Application Notes | ELISA The APE05571 antibody was tested for the ability to bind cell surface DDR in mouse serum by an ELISA assay. The antibody was incubated in normal mouse serum for 1, 4, or 7 days at 37°C. hDDR1-avi-his was coated on plates at 1 μg/m overnight at 4° C and washed three times in PBST buffer. Plates were then blocked with 3% BSA/PBS (200 μl/well) for one hour. The antibody APE05571 was then diluted, added to the washed plates, and incubated for two hours at room temperature. Bound antibodies were detected with goat anti HuIgG (H+L)-(HRP) at a dilution of 1:10,000. After washing the plates, 100 μl of TMB substrate was added to detect antibody, and plates were read at 450 nm. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | DDR1 |
| Alternative Name | CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A; epithelial discoidin domain-containing receptor 1; CD167 antigen-like family member A; PTK3A protein tyrosine kinase 3A; cell adhesion kinase; mammary carcinoma kinase 10; neuroepithelial tyrosine kinase; neurotrophic tyrosine kinase, receptor, type 4; protein-tyrosine kinase RTK-6; tyrosine kinase DDR; tyrosine-protein kinase CAK |
| Gene ID | 780 |
| UniProt | Q08345 |
| Research Area | Neuroscience; Cancer Research |
