| Description | This product is a monoclonal antibody that is capable of binding to deamidated gluten proteins and has no cross-reaction with the non-deamidated gluten proteins. The antibody selectively recognizes the deamidated gluten proteins, deamidated α-gliadin, deamidated γ-gliadin, deamidated gliadin, deamidated ω-5 gliadin and deamidated low-molecular-weight glutenin. The antibody is IgG1 isotype. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Cereal |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA |
| Application Notes | Competitive ELISA This assay based on the use of the LQPEEPFPEQC peptide conjugated to BSA (100 ng/well) and of the PEE antibody supernatant diluted to 1/4000 makes it possible to specifically detect deamidated gliadins at a concentration of between 1 and 8 ng/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Deamidated gluten protein |
| Alternative Name | Deamidated gluten protein |
