| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Dipeptidyl peptidase 4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Epitope | The antibody binds to peptides 6 (YSLRWISDHEYLY), 37 (YVKQWRHSYTASY), 55 (TWSPVGHKLAYVW), 79 (EEEVFSAYSALWW) and 132 (RISLQWLRRIQNY). |
| Affinity | KD = 0.2 nM, determined by surface plasmon resonance. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; DB; ELISA |
| Application Notes | The antibody is recommended for detection of DPP4 by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | DPP4 |
| Alternative Name | Dipeptidyl-Peptidase 4; Adenosine Deaminase Complexing Protein 2; Dipeptidylpeptidase IV (CD26, Adenosine Deaminase Complexing Protein 2); T-Cell Activation Antigen CD26; Dipeptidyl Peptidase IV; EC 3.4.14.5; DPP IV; ADCP-2; ADCP2; ADABP; TP103; CD26; Dipeptidylpeptidase 4; CD26 Antigen; DPPIV |
| Gene ID | 1803 |
| UniProt | P27487 |
| Research Area | Immunology |
| Related Disease | Diseases associated with expression of CD26 |
Figure 1 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in WB
Western blot analysis of V3S-0522-YC6578 (2 μg/mL) was performed by loading Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg
Figure 1 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in WB
Figure 2 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in DB
Dot Blot analysis of V3S-0522-YC6578 (2 μg/mL) was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.
Figure 2 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in DB
Figure 3 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in ELISA
ELISA analysis of V3S-0522-YC6578 was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). Then blocked with BSA, and incubated with V3S-0522-YC6578. The HRP-conjugated goat anti-human IgG as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in ELISA
Figure 4 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC6578 in non-reduced (Lane 1, 2 μg) and β-mercaptoethanol-reduced (Lane 2, 2 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 4 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in SDS-PAGE
Figure 5 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in SEC-HPLC
The purity of V3S-0522-YC6578 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 5 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in SEC-HPLC
