Recombinant Anti-DPP4 (aa 58-322) Antibody (V3S-0522-YC6578) (CAT#: V3S-0522-YC6578)

Figure 1 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in WB

Western blot analysis of V3S-0522-YC6578 (2 μg/mL) was performed by loading Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.

Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg

Figure 2 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in DB

Dot Blot analysis of V3S-0522-YC6578 (2 μg/mL) was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.

Figure 3 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in ELISA

ELISA analysis of V3S-0522-YC6578 was performed by coating with Recombinant Human DPP4/CD26 Protein (His Tag). Then blocked with BSA, and incubated with V3S-0522-YC6578. The HRP-conjugated goat anti-human IgG as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC6578 in non-reduced (Lane 1, 2 μg) and β-mercaptoethanol-reduced (Lane 2, 2 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 5 Anti-DPP4 Monoclonal Antibody (V3S-0522-YC6578) in SEC-HPLC

The purity of V3S-0522-YC6578 was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl