Sub Cat | Epitope | Isotype | Application | Affinity | |
---|---|---|---|---|---|
V3S-1124-YC272 | The antibody binds to peptides 29 (DYSISPDGQFILL), 30 (SISPDGQFILLEY) and 55 (TWSPVGHKLAYVW). | IgG1 | ELISA | 1.7 nM | |
V3S-0522-YC6582 | The antibody binds to peptides 29 (DYSISPDGQFILL), 30 (SISPDGQFILLEY), 55 (TWSPVGHKLAYVW) and 63 (IYVKIEPNLPSYR). | IgG1 | ELISA | 2.1 nM | |
V3S-0522-YC6576 | The antibody binds to peptides 6 (YSLRWISDHEYLY), 35 (LEYNYVKQWRHSY), 55 (TWSPVGHKLAYVW), 84 (LWWSPNGTFLAYA) and 132 (RISLQWLRRIQNY). | IgG1 | ELISA | 0.8 nM |
Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Dipeptidyl peptidase 4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human |
Epitope | The antibody binds to peptides 29 (DYSISPDGQFILL), 30 (SISPDGQFILLEY) and 55 (TWSPVGHKLAYVW). |
Affinity | KD = 1.7 nM, determined by surface plasmon resonance. |
Isotype | IgG1 |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
Application | ELISA |
Application Notes | The antibody is recommended for detection of DPP4 by ELISA assay. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | DPP4 |
Alternative Name | Dipeptidyl-Peptidase 4; Adenosine Deaminase Complexing Protein 2; Dipeptidylpeptidase IV (CD26, Adenosine Deaminase Complexing Protein 2); T-Cell Activation Antigen CD26; Dipeptidyl Peptidase IV; EC 3.4.14.5; DPP IV; ADCP-2; ADCP2; ADABP; TP103; CD26; Dipeptidylpeptidase 4; CD26 Antigen; DPPIV |
Gene ID | 1803 |
UniProt | P27487 |
Research Area | Immunology |
Related Disease | Diseases associated with expression of CD26 |