| Description | The mouse neutralizing antibody EEEV-5 recognizes the E2 glycoprotein of Eastern equine encephalitis virus (EEEV). It exhibited moderate to high levels of protection (40-100% survival rates) in mice assay. This antibody neutralizes infection at a post-attachment stage and efficiently blocked viral plasma membrane fusion. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Eastern equine encephalitis virus (EEEV) |
| Immunogen | SINV-EEEV |
| Epitope | The domain A (interacted with Lys71 and Lys74 of the three HS binding residues) |
| Neutralization Breath | The EEEV-5 had the lowest average occupancy of 45%. |
| Isotype | Mouse IgG2c |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Inhib; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EEEV E2 |
| Alternative Name | Eastern equine encephalitis virus E2 glycoprotein |
| Research Area | Infectious Disease |
| Related Disease | Febrile illness; Neurologic disease |
