| Description | This product is a mouse neutralizing antibody clone EEEV-58 specific for EEEV E2 glycoprotein. It recognizes an epitope in the "wing region" on E2 glycoprotein, a solvent exposed site at the distal tip of the A domain. It protected against death (70-100% survival) when administered as prophylaxis in vivo assay. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Eastern equine encephalitis virus (EEEV) |
| Immunogen | SINV-EEEV |
| Epitope | It recognizes an epitope in the "wing region" (residues 51-81) on E2, a solvent exposed site at the distal tip of the A domain. |
| Neutralization Breath | EEEV-58 had the highest average occupancy of 97%. |
| Isotype | Mouse IgG2c |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Inhib; Neut |
| Application Notes | For ELISA, MAb was diluted to specified concentrations and incubated for 1 h at 37°C with SINV-EEEV at an MOI of 0.01 for inhibition assay. Diluted mAb was added to virus adsorbed cells and incubated for 1 h at 4°C in neutralization assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EEEV E2 |
| Alternative Name | Eastern equine encephalitis virus E2 glycoprotein |
| Research Area | Infectious Disease |
| Related Disease | Febrile illness; Neurologic disease |
