Anti-EGFR Neutralizing Antibody (V3S-0522-YC6454) (CAT#: V3S-0522-YC6454)

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MSDS

COA

Sub Cat Immunogen Epitope Isotype Application Affinity  
V3S-1124-YC433 IgG Inhib 1.12 nM Inquiry
V3S-0522-YC7953 Human epidermal growth factor receptor IgG Block Inquiry
V3S-0522-YC8103 IgG WB; Neut; FuncS Inquiry
V3S-0622-YC1447 CHO cells transfected with a plasmid bearing a truncated form of EGFR cDNA IgG IF; IP; Neut; FuncS; ELISA; FC; ICC Inquiry
V3S-0622-YC1448 EGFR expressed on the surface of cells (A431 cells) IgG ELISA; IP; FC; FuncS; Neut; IF; ICC Inquiry
V3S-0622-YC1450 IgG ELISA; FC; IP; FuncS; IF; Neut; ICC Inquiry
V3S-0622-YC1453 IgG FC; IP; ELISA; Neut; FuncS; IF; WB Inquiry
V3S-0622-YC4421 IgG FuncS; IF; Neut; ELISA; FC; IP; ICC Inquiry
V3S-0622-YC4427 IgG ELISA; IP; FC; FuncS; Neut; IF; IHC Inquiry
V3S-0522-YC6188 Located in peptide:LYNPTTYQMD. IgG FC Inquiry
V3S-0522-YC6193 Located in peptide:CRNVSRGREC. IgG FC Inquiry
V3S-0522-YC6197 Located in peptide:CAHYIDGPHC. IgG FC Inquiry
V3S-0522-YC6202 Located in peptide:MGENNT. IgG FC Inquiry
V3S-0522-YC6184 Located in peptide: LVWKYADAG. IgG FC Inquiry
V3S-0622-YC140 In the CR1 domain (domain II), aa 287-302. IgG1 ELISA; FC; Inhib; FuncS 220 nM Inquiry
V3S-1022-YC309 In the C-terminus of EGFR. IgG2 Inhib Inquiry
V3S-0522-YC6035 In the extracellular domain of EGFR. IgG ELISA; WB; FC Inquiry
V3S-0622-YC142 In the extracellular domain. IgG1 kappa ELISA; FuncS 0.3 nM Inquiry
V3S-0622-YC143 In the extracellular domain. IgG1 kappa ELISA; FuncS 0.05 nM Inquiry
V3S-0622-YC141 In the L2 domain. IgG1 ELISA; FC; Inhib; FuncS 4 nM Inquiry
V3S-1022-YC306 In the N-terminus of EGFR. IgG1 Cyt; ELISA; FC 2.8 nM Inquiry
V3S-1022-YC311 In the N-terminus of EGFR. IgG IF Inquiry
V3S-0522-YC1490 IgG1 kappa ELISA; IHC; FC; IP; IF; FuncS Inquiry
V3S-0522-YC534 EGFRvIII PEP3-KLH Antigen The epitope is EEKKGNYVVT. IgG1 FuncS; ELISA; FC; IHC 67 nM Inquiry
V3S-0522-YC5850 IgG FuncS Inquiry
V3S-0522-YC5852 IgG ELISA; FuncS Inquiry
V3S-0522-YC5862 IgG ELISA Inquiry
V3S-0522-YC5877 IgG Internalization Assay Inquiry
V3S-0522-YC5883 IgG1 ELISA; FC Inquiry
V3S-0522-YC5884 IgG1 ELISA Inquiry
V3S-0522-YC6737 IgG1 ELISA Inquiry
V3S-0522-YC6846 IgG ELISA; WB Inquiry
V3S-0522-YC6922 IgG ELISA Inquiry
V3S-0522-YC7325 Human epidermal growth factor receptor IgG WB; ELISA; FuncS Inquiry
V3S-0522-YC7676 Human epidermal growth factor receptor IgG1 kappa FC Inquiry
V3S-0522-YC8104 IgG WB; ELISA; FuncS Inquiry
V3S-0522-YC8105 IgG1 WB; ELISA; FuncS Inquiry
V3S-0622-YC3229 An EGFR-expressing cell line IgG1 kappa ELISA 1.02 nM Inquiry
V3S-0622-YC3230 IgG1 kappa ELISA Inquiry
V3S-0622-YC3231 IgG ELISA Inquiry
V3S-0622-YC4424 IgG ELISA; IHC; FC; IP; IF; WB Inquiry
V3S-0723-FY52 IgG ELISA; FuncS Inquiry
V3S-0822-YC2922 IgG IF; FuncS Inquiry
V3S-0822-YC2923 IgG ELISA; DB; FC; Inhib Inquiry
V3S-1022-YC1676 IgG FC; ELISA Inquiry
V3S-1022-YC1677 IgG FC; ELISA Inquiry
V3S-1022-YC25 IgG FC Inquiry
V3S-1022-YC2875 IgG WB; ELISA Inquiry
V3S-1022-YC310 IgG2 Agonist Inquiry
V3S-1022-YC53 IgG FC Inquiry
V3S-1022-YC621 IgG ELISA; IHC; FC Inquiry
V3S-1022-YC625 IgG ELISA 750 nM Inquiry
V3S-1022-YC939 IgG Inhib; WB; FC 15.4 nM Inquiry
V3S-1022-YC941 IgG FC Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Epidermal growth factor receptor. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Affinity KD = 1.12 nM, determined by surface plasmon resonance.
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application Inhib
Application Notes The antibody is recommended for detection of EGFR by Inhib assay.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target EGFR
Alternative Name Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian)
Gene ID 1956
UniProt P00533
Research Area Signal Pathway
Related Disease Skin Cancer
For research use only, not directly for clinical use.
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