| Description | This product is a human monoclonal antibody that reacts with EGFR. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | FuncS; IF; Neut; ELISA; FC; IP; IHC |
| Application Notes | The antibody is recommended for detection of EGFR by FuncS, IF, Neut, ELISA, FC, IP, IHC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EGFR |
| Alternative Name | EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; |
| Gene ID | 1956 |
| UniProt | P00533 |
| Research Area | Signal Pathway |
Figure 1 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in SDS-PAGE.
SDS-PAGE analysis of V3S-0622-YC4426 in non-reduced (1.5 μg, Lane 1) and β-mercaptoethanol-reduced (1.5 μg, Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180 kDa.
Figure 1 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in SDS-PAGE.
Figure 2 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in SEC-HPLC.
The purity of V3S-0622-YC4426 was 98.2290% as determined by SEC-HPLC.
Figure 2 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in SEC-HPLC.
Figure 3 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in Western Blot.
Western blot analysis of V3S-0622-YC4426 was performed by loading human EGFR recombinant protein (Isoform vIII, His Tag, 39.3 kDa) onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a NC membrane and blocked with 5% skim milk for one hour. Membranes were probed with V3S-0622-YC4426 (2 ng/μL) and HRP goat anti-human IgG (H+L) as a secondary antibody (1: 2000) in turn. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 3 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in Western Blot.
Figure 4 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in ELISA.
ELISA analysis of V3S-0622-YC4426 was performed by coating with human EGFR recombinant protein (Isoform vIII, His Tag, 39.3 kDa). Then blocked with BSA and incubated with V3S-0622-YC4426. The HRP-conjugated goat anti-human IgG (H+L) as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in ELISA.
Figure 5 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in Dot Blot.
Dot blot analysis of V3S-0622-YC4426 was performed by loading human EGFR recombinant protein (Isoform vIII, His Tag, 39.3 kDa).
V3S-0622-YC4426 incubation concentration: 2 ng/μL.
The secondary antibody: HRP goat anti-human IgG (H+L)
Figure 5 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in Dot Blot.
