| Description | This product is a mouse/human chimeric antibody against EpCAM, designated as ChFU-MK-1, by fusing the VH and VL genes of FU-MK-1 to the human Cgamma1 and Ckappa genes, respectively. The ChFU-MK-1 antibody bound to human adenocarcinoma cells and competitively inhibited the binding of the parental FU-MK-1 to the adenocarcinoma cells. ChFU-MK-1 also showed a potent antibody-dependent cell-mediated cytotoxicity (ADCC) with human peripheral blood mononuclear cells as effectors against the adenocarcinoma cells. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human gastric adenocarcinoma |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FC; WB; ADCC |
| Application Notes | The antibody is recommended for detection of EPCAM by ELISA, FC, WB (1/1000-1/5000), ADCC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EPCAM |
| Alternative Name | Tumor-associated antigen GA733-2; GA733-2; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial cell adhesion molecule; adenocarcinoma-associated antigen; cell surface glycoprotein Trop-1; epithelial glycoprotein 314; human epithelial glycoprotein-2; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); trophoblast cell surface antigen 1; tumor-associated calcium signal transducer 1 |
| Gene ID | 4072 |
| UniProt | P16422 |
| Research Area | Signal Pathway |
