EPCAM Specific Neutra™ Antibody Products

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Epithelial cell adhesion molecule (EPCAM) is a cancer-associated antigen, classified within a family that includes type I membrane proteins. EPCAM is widely expressed on gastrointestinal carcinoma cells and normal epithelial, serving as a calcium-independent cell adhesion molecule. This antigen is harnessed as a target for immunotherapy in the treatment of human carcinomas. Mutations in EPCAM have been associated with congenital tufting enteropathy.

Its Gene ID: 4072, UniProtKB ID: P16422, and OMIM ID: 185535.

EPCAM Related Signaling Pathways

EPCAM is a homophilic cell-cell adhesion glycoprotein widely recognized as a tumor antigen expressed on circulating tumor cells, cancer stem cells, and epithelial tumors. The EPCAM signaling pathway is intricately associated with the differentiation, adhesion, and proliferation of epithelial cancer cells. Functioning as a crucial signaling molecule, EPCAM governs four independent pathways, including the Wnt signaling pathway, the nPKC-dependent pathway, the ERas/AKT pathway, and more. These pathways play pivotal roles in cadherin-mediated adhesion, the activation of AKT, and the modulation of the Wnt signaling cascade.

Fig. 1 EPCAM signaling pathway. (Eyvazi, et al., 2018) Fig.1 EPCAM signaling pathway.¹

Roles of Anti-EPCAM Antibodies in Cancer Treatment

The heightened expression of EPCAM in epithelial carcinomas compared to normal epithelia positions it as a promising candidate for targeted cancer therapies. Moreover, the exclusive localization of EPCAM in the basolateral membrane of normal epithelia poses a challenge for therapeutic agent accessibility. Recent decades have witnessed significant progress in protein engineering, bioinformatics, peptide design, and recombinant technology, resulting in the development of diverse therapeutic antibodies and peptides targeting various antigens. Consequently, several monoclonal antibodies (mAbs) directed against EPCAM have been introduced, emphasizing their potential roles in cancer treatment.

Cancer-specific targeting: unveiling the potential of anti-EPCAM mAb

EPCAM was initially recognized as a cancer-specific cell surface antigen through a functional screening approach involving the injection of cancer cells into mice for antibody generation. Anti-EPCAM mAbs exhibit anti-tumor activity by activating various endogenous cytotoxic mechanisms such as antibody-dependent cell-mediated cytotoxicity (ADCC). Additionally, they have the potential to induce a host-idiotype antibody response. Moreover, if the antibody is internalized, the activation of cytotoxic T cells will be not initiated. Creative Biolabs offers Recombinant Anti-EPCAM Antibody (V3S-0622-YC150) for detecting ADCC.

Fig. 2 Mechanism of action of EpCAM antibodies. (Macdonald, et al., 2018) Fig.2 Mechanism of action of EpCAM antibodies.²

Other applications of anti-EPCAM antibodies for comprehensive cancer research

Monoclonal antibodies targeting EpCAM are extensively employed for the isolation of circulating tumor cells (CTCs), serving as valuable indicators of micro-metastasis and offering crucial prognostic insights to assess the efficacy of therapeutic interventions. Additionally, these anti-EpCAM mAbs find utility in immunohistochemical analysis when investigating cancer tissues such as colorectal carcinoma tissues. Creative Biolabs presents Recombinant Anti-EPCAM Antibody (V3S-0522-YC126) and Recombinant Anti-EPCAM Antibody (V3S-0522-YC128) tailored for your IHC research endeavors, exemplifying our dedication to advancing innovative solutions in the field.

Creative Biolabs offers a diverse array of over 40 meticulously crafted anti-EPCAM antibody products specifically designed for advancing research in therapeutic strategies targeting various forms of cancer and other diseases. Additionally, we actively welcome inquiries pertaining to the personalized customization of anti-EPCAM bispecific antibodies, underscoring our commitment to fostering innovative solutions in the realm of biomedical research.

REFERENCES

Eyvazi, Shirin, et al. "Antibody based EpCAM targeted therapy of cancer, review and update." Current Cancer Drug Targets 18.9 (2018): 857-868.
Macdonald, Joanna, et al. "EpCAM immunotherapy versus specific targeted delivery of drugs." Cancers 10.1 (2018): 19.

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