| Description | This product is a recombinant mouse antibody provided by CreativeBiolabs. The antibody is specific for Epithelial Cell Adhesion Molecule, this gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. It can be used for EPCAM detection in Enzyme-Linked Immunosorbent Assay, Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant human EPCAM immune BALB/c mouse |
| Isotype | IgG |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EPCAM |
| Alternative Name | Epithelial Cell Adhesion Molecule; Tumor-Associated Calcium Signal Transducer 1; Major Gastrointestinal Tumor-Associated Protein GA733-2; Adenocarcinoma-Associated Antigen; Cell Surface Glycoprotein Trop-1; Epithelial Glycoprotein 314; TACSTD1; EGP314; MIC18; TROP1; M4S1; KSA; Membrane Component, Chromosome 4, Surface Marker (35kD Glycoprotein); Antigen Identified By Monoclonal Antibody AUA1; Human Epithelial Glycoprotein-2; Epithelial Cell Surface Antigen |
| Gene ID | 4072 |
| UniProt | P16422 |
| Research Area | Signal Pathway |
