| Description | This product is a mouse monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing human erb-b2 receptor tyrosine kinase 2 (ERBB2). It can be used for ERBB2 detection in Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry (FC). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Isotype | IgG2a kappa |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A or G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB; ELISA; FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-ERBB2 Monoclonal Antibody (V3S-0822-YC3125) in ELISA
ELISA analysis of V3S-0822-YC3125 was performed by coating with Recombinant Human ERBB2 Protein. Then blocked with BSA, and incubated with Anti-Human ERBB2 Antibody (V3S-0822-YC3125). The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-ERBB2 Monoclonal Antibody (V3S-0822-YC3125) in ELISA
Figure 2 Anti-ERBB2 Monoclonal Antibody (V3S-0822-YC3125) in WB
Western blot analysis of V3S-0822-YC3125 was performed by loading Recombinant Human ERBB2 Protein (lane 1, Non-Reducing Antigen, 0.2μg; lane 2, Reducing Antigen, 0.2μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0822-YC3125 and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 2 Anti-ERBB2 Monoclonal Antibody (V3S-0822-YC3125) in WB
Figure 3 Anti-ERBB2 Monoclonal Antibody (V3S-0822-YC3125) in SDS-PAGE
SDS-PAGE analysis of V3S-0822-YC3125 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
Figure 3 Anti-ERBB2 Monoclonal Antibody (V3S-0822-YC3125) in SDS-PAGE
