Anti-ESR1 (aa 247-261) Neutralizing Antibody (V3S-0822-YC1115) (CAT#: V3S-0822-YC1115)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a mouse monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing estrogen receptor 1. It can be used for ESR1 detection in Blocking (Block), Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Frozen (IHC-Fr), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Mouse
Target Species Human, Mouse, Rat, Fish, Sheep, Cattle, Avian
Immunogen Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.
Epitope in the region of aa 247-261.
Isotype IgG1

Property

Conjugation Unconjugated
Purity >95%, determined by SDS-PAGE
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application Block; FC; ICC; IF; IHC; IHC-Fr; IHC-P; IP; WB

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target ESR1
Alternative Name ESR1; estrogen receptor 1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Gene ID 2099
UniProt P03372
Research Area Signal Pathway; Neuroscience
For research use only, not directly for clinical use.
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