| Description | The mouse antibody 12B2 recognizes the Fusion glycoprotein (F glycoprotein) of Nipah virus (NiV) and Hendra virus (HeV). 12B2 recognize a prefusion-specific, conserved quaternary epitope and locks F in its prefusion conformation. It binds with high affinity to NiV F and broadly neutralizes NiV by blocking membrane fusion. It also broadly inhibited NiV-M, NiV-B and HeV. This product has been detected using Enzyme-linked immunosorbent assay, Bio-layer interferometry and Inhibition assay. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Nipah virus (NiV), Hendra virus (HeV) |
| Immunogen | Prefusion stabilized NiV F ectodomain trimer |
| Epitope | A conserved epitope including the oligosaccharide at position Asn67. |
| Affinity | 27.7 nM (NiV F) 5.35 nM (HeV F) |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Inhib |
| Application Notes | Bio-layer interferometry (BLI): The equilibrium dissociation constants (Kd) of 12B2 to NiV F or HeV F is 27.7 and 5.35 nM, respectively. Inhib: The Ab broadly inhibited NiV-M, NiV-B and HeV with IC50 ranging between 0.4-3.6 µg/mL for 12B2-h12B2. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | F glycoprotein |
| Alternative Name | Fusion glycoprotein |
| Research Area | Infectious Disease |
| Related Disease | Respiratory illness, severe encephalitis |
