| Description | This product is a human monoclonal antibody that reacts with F11. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | Inhib; ELISA; FC |
| Application Notes | The antibody is recommended for detection of F11 by Inhib, ELISA, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-F11 Monoclonal Antibody (V3S-0622-YC1556) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC1556 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
Figure 1 Anti-F11 Monoclonal Antibody (V3S-0622-YC1556) in SDS-PAGE
Figure 2 Anti-F11 Monoclonal Antibody (V3S-0622-YC1556) in SEC-HPLC
The purity of V3S-0622-YC1556 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Anti-F11 Monoclonal Antibody (V3S-0622-YC1556) in SEC-HPLC
Figure 3 Anti-F11 Monoclonal Antibody (V3S-0622-YC1556) in ELISA
ELISA analysis of V3S-0622-YC1556 was performed by coating with human F11, His Tag (1 µg/ml). Then blocked with BSA and incubated with V3S-0622-YC1556. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-F11 Monoclonal Antibody (V3S-0622-YC1556) in ELISA
