| Description | This product is a human monoclonal antibody that reacts with FCRN. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Soluble human FcRn (for panning) |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FuncS |
| Application Notes | The antibody is recommended for detection of FCRN by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FCRN |
| Alternative Name | FcRn; Fc receptor FcRn |
| Research Area | Cell Biology |
Figure 1 Recombinant Anti-FCRN Monoclonal Antibody in WB.
Western blot analysis of V3S-0622-YC1604 was performed by loading Human FCRN Protein.
Figure 1 Recombinant Anti-FCRN Monoclonal Antibody in WB.
Figure 2 Recombinant Anti-FCRN Monoclonal Antibody in ELISA.
ELISA analysis of V3S-0622-YC1604 was performed by coating with Human FCRN Protein.
Figure 2 Recombinant Anti-FCRN Monoclonal Antibody in ELISA.
Figure 3 Recombinant Anti-FCRN Monoclonal Antibody in Dot Blot.
Dot Blot analysis of V3S-0622-YC1604 was performed by coating with Human FCRN Protein.
Figure 3 Recombinant Anti-FCRN Monoclonal Antibody in Dot Blot.
