Recombinant Anti-GNRHR (aa 1-29) Antibody (V3S-1022-YC2323) (CAT#: V3S-1022-YC2323)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for Gonadotropin Releasing Hormone Receptor. It can be used for Gonadotropin Releasing Hormone Receptor detection in Enzyme-Linked Immunosorbent Assay, Western Blot, Immunohistochemistry and Immunofluorescence. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Synthesized oligopeptides corresponding to amino acid residues 1-29 in the extracellular domains of human GnRH receptor conjugated to keyhole limpet hemocyanin (KLH)
Epitope The epitope is in the aa 1-29 of the extracellular domain of the human GnRH receptor.
Affinity The affinity is in nanomolar range, about 2.0 nM, determined by surface plasmon resonance.
Isotype IgG

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA; WB; IHC; IF

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target GNRHR
Alternative Name GNRHR; gonadotropin-releasing hormone receptor; HH7; GRHR; LRHR; LHRHR; GNRHR1; gnRH-R; gnRH receptor; luliberin receptor; type I GnRH receptor; leutinizing-releasing hormone receptor; leutinizing hormone releasing horomone receptor; gonadotropin-releasing hormone (type 1) receptor 1
Gene ID 2798
UniProt P30968
Research Area Cell Biology
Related Disease Cancer
For research use only, not directly for clinical use.
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