| Description | This is an antagonist antibody that specifically reacts with human HAVCR2 (TIM-3) but does not murine HAVCR2. It effectively binds to activated T cells in both humans and marmoset monkeys, but not as strongly to macaques (cynomolgus and rhesus) monkey T cells. Additionally, this antibody can block the interaction of TIM-3 with three of its ligands. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Monkey |
| Immunogen | His-tagged recombinant human TIM-3 extracellular domain (ECD) protein |
| Epitope | Extracellular domain |
| Neutralization Mechanism | The antagonistic anti-TIM-3 antibody blocks ligand binding to abolish T cell inhibition, activate antigen-specific T cells, and improve anti-tumor immunity. |
| IC50 | 7.46 ± 0.052 nM |
| EC50 | 6.7 ± 8.7 nM |
| Affinity | KD = 2.3 nM (Human) KD = 11.8 nM (Cynomolgus) KD = 10.8 nM (Marmoset) |
| Isotype | Human IgG2 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Antagonist; Block; FC |
| Application Notes | In a competition-based ELISA, the antibody M6903, but not the isotype control antibody, partially inhibited the binding of huTIM-3-biotin to recombinant human Gal-9 (rhGal-9) in a concentration-dependent manner. The IC₅₀ value for this inhibition was determined to be 7.46 ± 0.052 nM. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HAVCR2 |
| Alternative Name | TIM3; CD366; KIM-3; SPTCL; TIMD3; Tim-3; TIMD-3; HAVcr-2; hepatitis A virus cellular receptor 2; T cell immunoglobulin mucin 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; kidney injury molecule-3 |
| Gene ID | 84868 |
| UniProt | Q8TDQ0 |
| Research Area | Immunology |
