Recombinant Anti-His Tag Antibody (V3S-0522-YC7376) (CAT#: V3S-0522-YC7376)

Figure 1 Anti-His Tag Monoclonal Antibody (V3S-0522-YC7376) in WB

Western blot analysis of V3S-0522-YC7376 was performed by loading a protein with His tag onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC7376 and HRP goat anti-mouse IgG (H+L) as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.6 μg)

Figure 2 Anti-His Tag Monoclonal Antibody (V3S-0522-YC7376) in DB

Dot Blot analysis of V3S-0522-YC7376 was performed by coating with a protein with His Tag.

V3S-0522-YC7376 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG (H+L) as a secondary antibody (1: 2000)
NC: Negative control PC: Positive control

Figure 3 Anti-His Tag Monoclonal Antibody (V3S-0522-YC7376) in ELISA

ELISA analysis of V3S-0522-YC7376 was performed by coating with a protein with His Tag. Then blocked with BSA and incubated with V3S-0522-YC7376. The HRP-conjugated goat anti-Mouse IgG (H+L) as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Recombinant Anti-His Tag Antibody (V3S-0522-YC7376) Fab Fragment in SDS-PAGE.

SDS-PAGE analysis of V3S-0522-YC7376 Fab fragment in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrated as about 25 kDa. And non-reduced protein migrated about 35-48 kDa.

Figure 5 Recombinant Anti-His Tag Antibody (V3S-0522-YC7376) Fab Fragment in Western Blot.

Western blot analysis of V3S-0522-YC7376 Fab fragment was performed by loading a protein with His tag onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with the Fab and HRP goat anti-mouse IgG (H+L) as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 6 Recombinant Anti-His Tag Antibody (V3S-0522-YC7376) Fab Fragment in ELISA.

ELISA analysis of V3S-0522-YC7376 Fab fragment was performed by coating with a protein with His Tag. Then blocked with BSA and incubated with the Fab. The HRP-conjugated goat anti-mouse IgG (H+L) as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 7 Recombinant Anti-His Tag Antibody (V3S-0522-YC7376) Fab Fragment in Dot Blot.

Dot blot analysis of V3S-0522-YC7376 Fab fragment was performed by coating with a protein with His Tag.
V3S-0522-YC7376 Fab fragment incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG (H+L) as a secondary antibody (1: 5000)
NC: Negative control PC: Positive control