| Description | This antibody is a mouse nuetralizing antibody clone 5F, which binding Human immunodeficiencyvirus-1 reverse transcriptase (HIV-1 RT). It is reactive with the p66 and p51 subunits of HIV-1 RT, indicating that it bounds to the polymerase domain of the enzyme. This antibody is capable of strongly inhibiting the RNA-dependent DNA polymerase activity of HIV-1 RT. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | HIV-1 |
| Immunogen | Infectious vaccinia virus recombinant (WRRT) expressing the RT of HIV-1 |
| Epitope | Its epitope is located within the region spanning amino acids 252-335 in the HIV-1 RT sequence, which contains the sequence of the thumb subdomain. |
| IC50 | 11 nM |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; SPR; Inhib |
| Application Notes | ELISA: 2 µg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 RT |
| Alternative Name | Human immunodeficiency virus type 1 reverse transcriptase |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
