| Description | 7C4 is a neutralizing antibody targeting the RT of HIV-1, derived from mouse. 7C4 interferes with the interaction between RT and the template primer and strongly inhibits the RNA-dependent polymerase activity of HIV-1 RT. This product has been determined by Enzyme-linked immunosorbent assay, Surface plasmon resonance and Inhibition assay. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | HIV-1 (IIIB, Bru and IMS-1) |
| Immunogen | Infectious vaccinia virus recombinant (WRRT) expressing the RT of HIV-1 |
| Epitope | The epitope recognized by 7C4 exists in the region of the template primer-binding sites (or the 'helix clump') of RT. |
| IC50 | 42 nM |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; SPR; Inhib |
| Application Notes | ELISA: After washing, 50 µl of 2 µg/mL IgG was added to each well and incubated for 1 h. Surface plasmon resonance (SPR): The resonance unit from the chip incubated with different concentrations of antibody after washing with HBS buffer was recorded. Inhibition assay: 7C4 Fab fragments induced 95% inhibition of RT activity at 400 nM. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 RT |
| Alternative Name | Human immunodeficiency virus type 1 reverse transcriptase |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
