| Description | The antibody is a human neutralizing antibody that binds to the human metapneumovirus fusion glycoprotein (hMPV F protein). It recognizes a site of vulnerability near the F trimer apex. It has potent neutralizing ability against hMPV and it potently neutralized multiple hMPV isolates. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | hMPV |
| Cross Reactivity | hMPV-A1, hMPV-A2, hMPV-B1, hMPV-B2 |
| Epitope | A site of vulnerability at antigenic site zero (Ø) near the hMPV F trimer apex. |
| Neutralization Mechanism | It binds to the hMPV F protein and recognizes a site of vulnerability near the F trimer apex. |
| IC50 | 39 ng/mL (hMPV-A1), 14 ng/mL(hMPV-A2), 15 ng/mL (hMPV-B1), 1 ng/mL (hMPV-B2) |
| Affinity | KD = 0.279 nM (hMPV-A1 preF) KD = 1.55 nM (hMPV-A1 postF) KD = 0.374 nM (hMPV-B2 preF) |
| Isotype | IgG |
| Expression Species | HEK293F or CHO cell |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Neut |
| Application Notes | Neutralization assay (Neut): The IC₅₀ value of this antibody against hMPV F protein is 0.039 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | hMPV preF |
| Alternative Name | Human metapneumovirus fusion glycoprotein; preF; fusion (F) protein; pre-fusion; hMPV |
| Research Area | Infectious Disease |
| Related Disease | Respiratory disease |
