| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Herpes simplex virus-1 envelope glycoprotein D. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Herpes Simplex Virus 1 (HSV1) |
| Isotype | IgG1, λ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of HSV1 gD by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-HSV-1 gD Monoclonal Antibody (V3S-0522-YC4181) in ELISA
ELISA analysis of V3S-0522-YC4181 was performed by coating with recombinant human herpesvirus 1 Envelope glycoprotein D (gD). Then blocked with BSA and incubated with Anti-HSV-1 gD Antibody (64-863) (V3S-0522-YC4181). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-HSV-1 gD Monoclonal Antibody (V3S-0522-YC4181) in ELISA
